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Bacterial Alkaloid Biosynthesis off the Beaten Path: Compartmentalization and Non-Enzymatic Transformations in Non-Canonical Alkaloid Biosynthesis

CORDIS · observation · Publication date unknown

atic transformations, thus defying the central dogma of NP biosynthesis. Bacteria likely employ specialized micro-compartments to facilitate these spontaneous reactions. The ComBiNE team will develop machine learning-based genome mining algorithms to systematically identify and characterize alkaloid BGCs that currently elude detection. These non-canonical pathways biosynthesize alkaloids independent of the ribosome and non-ribosomal peptide synthetases. We will focus on alkaloids that undergo non-enzymatic transformations in bacterial micro-compartments. We will establish a model system to study the level of compartmentalization necessary to facilitate spontaneous reactions. Insights gained from these studies will be used to engineer NP and primary metabolic pathways for the non-enzymatic fusion of tailor-made NPs or primary metabolites with complementary reactivity. Spontaneous reactions in bacterial micro-compartments can be harnessed for the fusion of two NPs with different targets to create bispecific chimeras to combat drug resistance or to fuse bioactive and homing components t

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recordType

award

status

SIGNED

region

EU

value

1499998

unit

EUR