SOURCE-LINKED INTELLIGENCE

Rewiring gene regulatory circuits to enhance central nervous system repair

CORDIS · observation · Publication date unknown

g on innovative technologies, some of which that I developed, we will identify injury-responsive enhancer elements in the mouse spinal cord with single cell and spatiotemporal resolution. Then, using machine learning, we will decode the rules of injury-sensing DNA elements to design synthetic injury-responsive enhancers for precise gene expression control in neural stem cells. Finally, we will use synthetic enhancers in therapeutically relevant gene delivery systems to rewire gene circuits in order to promote the recruitment resident stem cells for cell replacement through the reactivation of developmental genes that would otherwise remain silent. The proposed research will uncover basic principles of gene regulation after CNS injury and open new avenues for the design of smart gene therapies for regenerative medicine using synthetic regeneration enhancers. Spinal cord; Mouse; Single cell genomics; ATAC-seq; machine learning; enhancers; gene therapy; reprogramming.

Read original source ↗ Open in workspace

recordType

award

status

SIGNED

region

EU

value

1500000

unit

EUR